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  2. Biology
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IB Biology question bank

Augment’s own questions for Biology, written in the style of IB exams, each with its markscheme. They are not past papers. One from each topic is below to try.

  • IB Biology
  • SL and HL
  • 1,440 questions

What the Biology question bank holds

Every question has a markscheme.

Exam-style questions
1,440
For both SL and HL
830
HL only
610
Multiple choice
800
Counted on 29 October 2026

Biology questions by paper

The paper each question is written for, so you can practice one paper at a time.

  1. Paper 1AMultiple-choice questions.800 questions
  2. Paper 1BData-based questions on experimental work.200 questions
  3. Paper 2Short-answer and extended-response questions.440 questions

Biology questions by topic

Each topic in syllabus order, with its subtopics. Lines with notes link to them. Under each topic is one question to try, its markscheme folded until you open it.

A Unity and diversity

333 questions
  1. A1.1Water39 questions
  2. A1.2Nucleic acids42 questions
  3. A2.1Origins of cellsHL only38 questions
  4. A2.2Cell structure39 questions
  5. A2.3VirusesHL only36 questions
  6. A3.1Diversity of organisms40 questions
  7. A3.2Classification and cladisticsHL only36 questions
  8. A4.1Evolution and speciation43 questions
  9. A4.2Conservation of biodiversity37 questions
One to try · BIO-000011 mark

Why is water described as the medium for life?

  1. A

    Water is the only molecule in cells that can form hydrogen bonds

  2. B

    The first cells originated in water and most processes of life still occur in water

  3. C

    Water provides the energy needed for most metabolic reactions

  4. D

    Water is the most abundant organic molecule in living cells

The answer is B.

Explanation

The first cells are thought to have arisen in water, and most metabolic processes still take place in aqueous solution, so water is the medium for life. A is wrong because many other molecules (DNA bases, proteins, sugars) form hydrogen bonds. C confuses the medium with an energy source: water releases no usable energy. D is wrong because water is an inorganic molecule.

B Form and function

382 questions
  1. B1.1Carbohydrates and lipids43 questions
  2. B1.2Proteins40 questions
  3. B2.1Membranes and membrane transport52 questions
  4. B2.2Organelles and compartmentalisation39 questions
  5. B2.3Cell specialisation39 questions
  6. B3.1Gas exchange44 questions
  7. B3.2Transport45 questions
  8. B3.3Muscle and motilityHL only36 questions
  9. B4.1Adaptation to environment36 questions
  10. B4.2Ecological niches38 questions
One to try · BIO-00109HL only · 1 mark

Which row describes the changes in the bands of a sarcomere when a skeletal muscle fibre contracts?

Row

A band

I band

H zone

A

shorter

shorter

shorter

B

unchanged

unchanged

shorter

C

unchanged

shorter

shorter

D

shorter

unchanged

unchanged

  1. A

    A band shorter; I band shorter; H zone shorter

  2. B

    A band unchanged; I band unchanged; H zone shorter

  3. C

    A band unchanged; I band shorter; H zone shorter

  4. D

    A band shorter; I band unchanged; H zone unchanged

The answer is C.

Explanation

The A band is the length of the myosin (thick) filaments, which do not change length, so it stays the same. As actin filaments slide towards the centre, the regions containing only actin (I band) or only myosin (H zone) both narrow. Row A assumes the filaments themselves shorten; row B forgets that the I band narrows as the Z lines move closer to the thick filaments.

C Interaction and interdependence

362 questions
  1. C1.1Enzymes and metabolism43 questions
  2. C1.2Cell respiration44 questions
  3. C1.3Photosynthesis42 questions
  4. C2.1Chemical signallingHL only39 questions
  5. C2.2Neural signalling42 questions
  6. C3.1Integration of body systems40 questions
  7. C3.2Defence against disease44 questions
  8. C4.1Populations and communities42 questions
  9. C4.2Transfers of energy and matter43 questions
One to try · BIO-000371 mark

What is the main benefit to a cell of having enzymes that act as catalysts?

  1. A

    Reactions can proceed at a fast enough rate at the temperature of the cell

  2. B

    The enzymes supply the energy needed for reactions to occur

  3. C

    More product is formed when the reaction reaches equilibrium

  4. D

    The enzymes raise the temperature of the cytoplasm so that molecules collide more often

The answer is A.

Explanation

Catalysts increase the rate of reactions without being used up, so metabolic reactions happen quickly enough to sustain life at the relatively low temperatures found in cells. Enzymes do not supply energy (B); they lower the activation energy. They do not change the amount of product at equilibrium (C), and they do not heat the cell (D).

D Continuity and change

462 questions
  1. D1.1DNA replication41 questions
  2. D1.2Protein synthesis47 questions
  3. D1.3Mutations and gene editing42 questions
  4. D2.1Cell and nuclear division45 questions
  5. D2.2Gene expressionHL only37 questions
  6. D2.3Water potential36 questions
  7. D3.1Reproduction39 questions
  8. D3.2Inheritance37 questions
  9. D3.3Homeostasis46 questions
  10. D4.1Natural selection48 questions
  11. D4.2Stability and change37 questions
  12. D4.3Climate change38 questions
One to try · BIO-008651 mark

Which process in a multicellular organism depends on DNA replication?

  1. A

    Synthesis of insulin by cells in the pancreas

  2. B

    Production of mRNA from a gene in a liver cell

  3. C

    Replacement of worn-out cells lining the small intestine

  4. D

    Secretion of digestive enzymes by mature gland cells

The answer is C.

Explanation

New cells for growth and tissue replacement are produced by cell division, and each new cell needs an exact copy of the DNA, so the DNA must be replicated first. A and D involve translation and secretion of proteins, and B is transcription; none of these requires the DNA to be copied.

On this page

  • What it holds
  • By paper
  • A Unity and diversity
  • B Form and function
  • C Interaction and interdependence
  • D Continuity and change

Practice them all

In Augment you can filter the Biology questions by paper and difficulty, mark yourself against each markscheme and see which topics are holding up.

Open in Augment

More for IB Biology

  • IB BiologyThe Biology syllabus topic by topic, and every notes page.
  • Biology assessmentEach Biology paper, the internal assessment and grade boundaries.
  • Exam skills notesHow to tackle each Biology paper, and how each is marked.

Practice every Biology question

A free account opens the whole question bank, keeps a record of what you have marked, and builds worksheets from the topics you pick.

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